OBJECTIVE： To investigate the effects of Xinshao formula on the apoptosis of oxygen-glucose deprivation （OGD）-induced PC12 cells， and to investigate its mechanism. METHODS： PC12 cells were cultured by Na2S2O4 to establish OGD model. OGD-induced cells were divided into model group （blank medium）， nimodipine group （positive drug， 0.42 mg/L） and Xinshao formula low-concentration， medium-concentration and high-concentration groups [0.313， 0.625， 1.25 mg （crude drug）/L]； normal cells were included in normal control group （blank medium）. MTT assay was used to determine the survival rate of PC12 cells after treated with drugs for 4 h and then oxygen-glucose restoration for 4 h. The release of LDH was detected by colorimetric method. The apoptosis rate and mitochondrial membrane potential （MMP） were detected by flow cytometry. The protein expression of Caspase-3， Bax and Bcl-2 were examined by Western blot. RESULTS： Compared with normal control group， the survival rate， MMP， the protein expression of Bcl-2 and the ratio of Bcl-2/Bax were decreased significantly in model group； while the release of LDH， apoptosis rate， protein expression of Caspase-3 and Bax were increased significantly （P＜0.05 or P＜0.01）. Compared with model group， the survival rate， MMP， the protein expression of Bcl-2 and the ratio of Bcl-2/Bax were increased in Xinshao formula groups； while the release of LDH， apoptosis rate， protein expression of Caspase-3 and Bax were decreased. The above indexes had significant differences except for MMP， Bcl-2 and Bax in Xinshao formula low-concentration group （P＜0.05 or P＜0.01）. CONCLUSIONS： Xinshao formula can inhibit the apoptosis of OGD-induced PC12 cells， which may be related to reducing the release of LDH， keeping MMP stable and regulating the expression of apoptotic-related gene.