OBJECTIVE： To study the effects of Glycyrrhiza uralensis on endogenous metabolites levels of triptolide （TPL） and triptonide （TPN） in rats. METHODS： 160 rats were given G. uralensis [30 g （crude drug）/kg] intragastrically， 2 h later given TPN （2.8 mg/kg） intraperitoneally for consecutive 30 d. 24 h urine were collected for 30 days， and then endogenous metabolites were separated and identified by column chromatograph and spectrum technology. UPLC-Q-TOF/MS was used to determine the common metabolites after giving TPL. 60 rats were randomly divided into TPL blank group， TPL group， G. uralensis+TPL group， TPN blank group， TPN group， G. uralensis+TPN group. Route of administration and dosage of treatment groups were same as above groups. TPL blank group and TPN blank group were given corresponding solvent， once a day， for consecutive 7 d. UPLC-Q-TOF/MS was used to determine the endogenous metabolites of TPL and TPN in rats. RESULTS： Five endogenous metabolites were isolated and identified from urine of rats after given TPN， as 1-methylhydantoin， methyl 4-aminonicotinate， 3，4-dihydro-6-hydroxy-2（1H）-quinolinone， 2，6-quinolinediol， 2-quinolone， respectively. Those five metabolites were also found in urine of rats after giving TPN. 1-methylhydantoin in TPL group and 2，6-quinolinediol in TPN group were significantly increased， compared with TPL blank group or TPN blank group （P＜0.05）； while urine levels of 1-methylhydantoin and 2，6-quinolinediol decreased after TPL or TPN combining with G. uralensis， compared with TPL/TPN blank group， with no significant difference （P＞0.05）. CONCLUSIONS： The above five endogenous metabolites were isolated from rat urine after given TPL or TPN for the first time. The levels of 1-methylhydantoin after giving TPL and 2，6-quinolinediol in urine after giving TPN can be recovered by G. uralensis.