OBJECTIVE： To establish a HPLC method to determine DHMEQ intermediate N-（2-acetoxybenzoyl）-2，5-dimethoxyaniline（MA）. METHODS： The column was Utimate AQ-C18 with mobile phase of acetonitrile-0.05％ phosphoric acid （gradient elution） at a flow rate of 1 ml/min， detection wavelength was 230 nm， column temperature was 30℃， and the injection volume was 10 μl. RESULTS： The separation degrees of MA and the impurities were above 1.5， the number of theoretical plates （calculated with MA peaks） was no less than 5 000， retention time was about 41 min； the detection limits （the percentages of MA samples） of MA， methyl acetylsalicylate， SM2， SM1， MA-6， salicylic acid and MA-2 were 0.04％， 0.03％， 0.01％， 0.05％， 0.05％， 0.05％ and 0.05％； detection limits （the percentage of MA samples） were 0.01％， 0.01％， 0.004％， 0.02％， 0.02％， 0.02％ and 0.02％， respectively； the stability of solution and durability of method were good. CONCLUSIONS： The specificity， detection limit， and durability of the method are all in line with the requirements， which can be used for DHMEQ intermediate MA and its impurity.