OBJECTIVE： To investigate the pathogen distribution and drug resistance of burn patients in our hospital， and to provide reference for rational use of antimicrobial agents in the clinic. METHODS： 389 burn patients were selected from our hospital during Jan. 2013 to Dec. 2015 by simple random sampling， and then analyzed retrospectively in respects of pathogen culture， identification and the results of sensitivity tests. RESULTS： 678 clinical specimen were collected from 389 burn patients of our hospital， 564 strains of pathogens were detected， with positive rate of 83.19％. Of 564 pathogens， there were 367 stains of Gram-negative bacteria （65.07％）， mainly including Pseudomonas aeruginosa （158 strains）， Escherichia coli （67 strains）， Acinetobacter baumannii （36 strains）， Enterobacter cloacae （31 strains）； there were 177 strains of Gram-positive bacteria （31.38％）， mainly including Staphylococcus aureus （81 strains）， Enterococcus （44 strains） and Staphylococcus epidermidis （26 strains）； there were 20 strains of fungus （3.55％）， mainly including Candida albicans （13 strains）. There were 42 strains of ESBLs E. coli （62.69％） and 11 strains of ESBLs Klebsiella pneumoniae （40.74％）. Gram-negative bacteria were highly resistant to aminoglycosides， β-lactamase， tetracycline and cephalosporin. P. aeruginosa was sensitive to colistin sulphate. E. coli， E. cloacae and K. pneumoniae were sensitive to imipenem， A. baumannii was sensitive to meropenem， and their resistant rates were 0. Gram-positive bacteria were highly resistant to many common antimicrobial agents； S. aureus was sensitive to vancomycin， S. epidermidis sensitive to vancomycin， teicoplanin and minocycline， and their resistant rates were 0. Resistant rates of C. albicans and C. tropicalis to amphotericin B and 5-fluorocytosine were ≤5％. CONCLUSIONS： Main pathogen of infection in burn patients of our hospital is Gram-negative bacteria， mainly being P. aeruginosa. Producing enzymes and drug resistance of main pathogens are serious. It is necessary to standardize clinical application of antimicrobial agents and choose antimicrobial agents rationally according to etiological examination and clinical symptoms.