OBJECTIVE： To separate and identify the chemical constituents of Broussonetia papyrifera fruits. METHODS： 95％ ethanol extracts from B. papyrifera fruits were extracted with different polar solvents as aether petroleum and ethyl acetate in turn. The extract parts were isolated and purified by various chromatographic methods including silica gel， ODS， Sephadex LH-20 and recrystallization. The chemical structure of them were identified according to physiochemical property and spectral data. RESULTS： Eleven compounds isolated from 95％ ethanol extracts were identified as compound 1 （physcion）， compound 2 （apigenin）， compound 3 （apigenin-7-O-β-D-glucopyranoside），compound 4 （quercetin）， compound 5 （β-daucosterol），compound 6 （daucosterol palmitate），compound 7 （palmitic acid ethyl ester），compound 8 （δ-tocopherol），compound 9 （linoleic acid），compound 10 （phytol） and compound 11 （palmitic acid）. Compound 2-4 and compound 7-11 were all isolated from B. papyrifera fruits for the first time. CONCLUSIONS： The study further confirms the chemical constituents of B. papyrifera fruits， and can provide scientific evidence for material base of pharmacological activity of B. papyrifera.