OBJECTIVE： To establish a method for the content determination of L（-）-Anabasine in different medicinal parts （stem， rhizome， fibrous root， rootlet） of Alangium chinense，and provide reference for its development and utilization. METHODS： HPLC was performed on the column of Thermo C18 with mobile phase of methanol-phosphate buffer solution （22 ∶ 78，V/V） at a flow rate of 1.0 ml/min， the detection wavelength was 259 nm， column temperature was 30 ℃； the injection volume was 10 μl. RESULTS： The linear range of L（-）-Anabasine was 0.020 62-0.257 80 μg（r＝0. 999 9）； the limit of quantitation was 1.7 ng， limit of detection was 0.5 ng； RSDs of precision， stability and reproducibility tests were lower than 2％； recovery was 97.38％-98.86％ （RSD＝0.6％，n＝6）. The content of L（-）-Anabasine in different medicinal parts was the fibrous root＞the rootlet＞the rhizome＞the stem. CONCLUSIONS： The method is simple， accurate， reproducible and specific， and suitable for the content determination of L（-）-Anabasine in different medicinal parts （stem， rhizome， fibrous root， rootlet） of A. Chinense.