OBJECTIVE： To establish a method for simultaneous determination of lysophosphatidyl choline （LPC） and lysophosphatidyl ethanolamine （LPE） in Nimodipine fat emulsion. METHODS： HPLC was performed on the column of Lichrosher Diol with detector of evaporative light scattering detector with mobile phase A of heptane - isopropyl alcohol solution （43 ∶ 57，V/V）， mobile phase B n-heptane - isopropyl alcohol - water （29.5 ∶ 59 ∶ 11.5， V/V/V） （gradient elution） at a flow rate of 1.5 ml/min， column temperature was 40 ℃， and the injection volume was 20 μl. RESULTS： The linear range was 0.020 0-0.400 0 mg/ml for LPC （r＝0.999 0） and 0.010 0-0.200 0 mg/ml for LPE （r＝0.999 6）， and the logarithm value of concentration and peak area showed good linear relationship； the limit of quantitation was 0.013 4 mg/ml for LPC and 0.013 0 mg/ml for LPE； the limit of detection was 0.007 8 mg/ml for LPC and 0.007 6 mg/ml for LPE； RSDs of precision， stability and reproducibility tests were lower than 2％； recoveries were 95.96％-100.63％ （RSD＝1.83％， n＝9） and 99.22％-101.76％ （RSD＝0.80％，n＝9）. CONCLUSIONS： The method is simple， and can be used for the determination of related substance in Nimodipine fat emulsion.