OBJECTIVE： To establish a method for the simultaneous determination of paeoniflorin， baicalin and imperatorin in Urticaria pill. METHODS： HPLC was performed on the column of Alltima C18 with mobile phase of methanol-water-Phosphoric acid （55 ∶ 45 ∶ 0.2，V/V/V） at a flow rate of 1.0 ml/min， the detection wavelength was 230 nm for paeoniflorin， 280 nm for baicalin and 300 nm for imperatorin， column temperature was 30 ℃， and the volume injection was 10 μl. RESULTS： The linear range was 5.40-54.0 μg/ml for paeoniflorin （r＝0.999 8），11.29-112.9 μg/ml for baicalin（r＝0.999 7） and 24.95-249.5 μg/ml for imperatorin （r＝0.999 9）， respectively； the linit of quantitation were 5.4、11.2、30.0 ng，the lirnit of detection were 1.8、2.8、7.5 ng； RSDs of precision， stability and reproducibility tests were lower than 2％； recoveries were 95.88％-98.33％ （RSD＝0.95％，n＝6），96.86％-99.96％（RSD＝1.20％，n＝6） and 98.07％-100.55％（RSD＝0.92％，n＝6）， respectively. CONCLUSIONS： The method is simple and accurate with strong specificity and good reproducibility， and can be used for the simultaneous determination of paeoniflorin， baicalin and imperatorin in Urticaria pill.