OBJECTIVE： To optimize the extraction and purification technology of total flavonoids from Engelhardia roxburghiana， and to establish the method for the content determination of 3 kinds of effective components. METHODS： Using the extraction transfer rate of astilbin as index， single factor test was used to investigate extraction solvent， extraction method， volume fraction of percolation solvent ethanol， percolation material-liquid ration， soaking time before percolation and percolation rate of extraction technology， and volume fraction of eluant ethanol in AB-8 resin purification technology. The contents of 3 effective components as astilbin， texifolin and engelitin in total flavonoids from E. roxburghiana were determined by HPLC. RESULTS： The optimal extraction technology was using 70％ ethanol as extraction and percolation solvent， percolation extraction， soaking for 8 h before percolation， percolation material-liquid ratio of 1 ∶ 16 （g/ml）， percolation rate of 30 ml/（min·kg）. The purification technology was diluting the solution to 0.5 g （crude drug）/ml with water， ethyl acetate extraction， dissolved extract with 50％ ethanol after evaporated to dryness， AB-8 resin for sampling， eluted with 50％ ethanol， concentrating and drying. In verification test， extraction transfer rate of astilbin was more than 80％ （RSD＝0.42％， n＝3）. The contents of astilbin， taxifolin and engeletin in total flavonoids from E. roxburghiana by purified were 57.94％， 3.72％ and 2.83％， respectively； the contents of 3 components accounted for 64.00％ of total flavonoids. CONCLUSIONS： The extraction and purification technology is stable， rational and reliable； the content determination method of 3 effective components in total flavonoids of E. roxburghiana is accurate， simple and producible.