OBJECTIVE： To compare the contents of loganic acid， swertiamarin， 6′ -O-β-D-glucosyl gentiopicroside， gentiopicroside， sweroside， isoorientin and isovitexin in wild and cultivated Gentiana officinalis， and to provide basis for rational use of G. officinalis. METHODS： UPLC method was adopted. The separation was performed on ACQUITY UPLC® BEH C18 column （50 mm×2.1 mm， 1.7 μm） with mobile phase consisted of methanol-0.04％ phosphoric acid （gradient elution） at the flow rate of 0.3 ml/min. The detection wavelength was set at 242 nm， and column temperature was 30 ℃. RESULTS： For loganic acid， swertiamarin， 6′ -O-β- D-glucosyl gentiopicroside， gentiopicroside， sweroside， isoorientin and isovitexin， a good linearity was obtained in the range of 2.1-537.1 μg， 1.05-270 μg， 0.92-236 μg， 11.1-2 830 μg， 0.75-192 μg， 0.167-102 μg， 0.216-52.80 μg （r≥0.999 5）， respectively. Their average recoveries were 97.72％-99.84％ （RSD≤3.39％， n＝6）. The contents of loganic acid， swertiamarin， 6′ -O-β-D-glucosyl gentiopicroside， gentiopicroside， sweroside and isoorientin in the wild sample were higher than in the cultivated； the content of isovitexin was lower than the cultivated， but there was no statistical significance （P＞0.05）. The sum of gentiopicroside and loganin acid content were all higher than 2.5％ in both wild and cultivated samples， and met the requirements of 2015 edition of Chinese Pharmacopoeia （first part）. CONCLUSIONS： The content difference of 7 indicative constituents in wild and cultivated G. officinalis is not statistically significant， and the indicative constituents of the pharmacopoeia is qualified.