OBJECTIVE: To establish a method for the evaluation of the uncertainty for the concentration calibration of Cerous sulfate titration solution (0.1 mol/L). METHODS: The sources of uncertainty of Cerous sulfate titration solution was analyzed, and measurement model was established to evaluate the components of uncertainty and calculate the expanded uncertainty. RESULTS: The expanded uncertainty of Cerous sulfate titration solution (0.1 mol/L) was 0.000 5 mol/L and the concentration was (0.101 2±0.000 5) mol/L, coverage factor k=2. CONCLUSIONS: The method is suitable for the uncertainty evaluation for the concentration calibration of Cerous sulfate titration solution.