OBJECTIVE： To establish a method for the content determination of berberine hydrochloride in Fufang huangge jiangtang tablet. METHODS： UPLC was performed on the column of Acquity UPLC BEH C18 with mobile phase of acetonitrile-0.1％ H3PO4（21 ∶ 79，V/V） at flow rate of 0.3 ml/min， the detection wavelength was 345 nm， column temperature was 30℃， and the volume injection was 1.0 μl. RESULTS: The linear range of berberine hydrochloride was 0.008 16-0.081 6 μg（r＝0.999 9）； RSDs of precision， stability and reproducibility were lower than 1％； average recovery was 97.05％-98.96％（RSD＝0.66％，n＝6）. CONCLUSIONS: The method is simple， precise， stablity and reproducible， and can be used for the quality control of Fufang huangge jiangtang tablet.