OBJECTIVE： To provide reference for the standardization of online pharmacies pharmacist service. METHODS： Literature research method， online entity investigation method and the method of mysterious customer were adopted to investigate 10 well-known online pharmacies， then described the analysis of current online pharmacies pharmacist service in China. RESULTS： There were 5 online pharmacies showing pharmacist service， but only 1 pharmacy could provide pharmacist registration certificate； on average each online pharmacy was allocated with 1.9 licensed pharmacists； only 2 online pharmacies provided 24 hours of pharmacist service； 8 online pharmacies provided more than 2 consulting method； messagebox would pop actively when clicking the “Buy” button in 6 online pharmacies； the correct rate of pharmacist’s guidance was less than 60％， only 20％ pharmacists could provide right explanation. CONCLUSIONS： The reasons of the lack of online pharmacies pharmacist service include online pharmacies imperfect laws and regulations and supervision， lack of licensed pharmacists quantity and quality is not high enough， online pharmacies， pharmacists consulting system is not mature. Online pharmacies can improve relevant laws and regulations， strengthen the government supervision， reasonable and effective use of licensed pharmacists resources， perfect the pharmacist online consulting service water system and improve the licensed pharmacists to improve pharmacists service function status.