OBJECTIVE： To establish a method for the simultaneous determination of chlorogenic acid， evodiamine， rutecarpine in different places of Evodia rutaecarpa. METHODS： UPLC was performed on the column of ACQUITY UPLC BEH C18 with mobile phase of acetonitrile-0.1％ Phosphoric acid aqueous solution （gradient elution） at a flow rate of 0.40 ml/min， the detection wavelength was 326 nm and 220 nm， column temperature was 30 ℃， and the volume injection was 2 μl. RESULTS： The linear range was 7.67-76.67 μg/ml for chlorogenic acid（r＝0.999 2），13.33-133.33 μg/ml for evodiamine（r＝0.999 7）and 13.33-133.33 μg/ml for rutecarpine（r＝0.999 8）； the limits of quantitation were 0.11 ng， 0.05 ng and 0.05 ng， the limits of detection were 0.03 ng， 0.01 ng and 0.01 ng，respectively； RSDs of precision， stability and reproducibility tests were lower than 3％； recoveries were 96.19％-101.90％ （RSD＝2.19％，n＝6），95.35％-101.16％（RSD＝2.27％，n＝6） and 95.92％-98.98％（RSD＝1.33％， n＝6）， respectively. CONCLUSIONS： The method is rapid and accurate， and suitable for the simultaneous determination of chlorogenic acid， evodiamine， rutecarpine in different places of E. rutaecarpa.