OBJECTIVE： To establish a method for the determination of content and content uniformity in Bisacodyl enteric-coated  tablet. METHODS： HPLC method was performed on the column of Agilent ZORBAX C18 with mobile phase of acetonitrile-20 mmol/L ammonium acetate （adjusted pH to 5.0 with acetic acid） （55 ∶ 45，V/V）， the detection wavelength was 265 nm， column temperature was 30 ℃， flow rate was 1.0 ml/min， and the volume injection was 20 μl. RESULTS： The linear range of bisacodyl was 50-1 000 μg/ml（r＝0.999 9）； RSDs of precision， stability and reproducibility tests were lower than 1％； recovery was 99.50％-101.17％（RSD=0.5％，n＝9）. CONCLUSIONS： The method is reproducible with high accuracy， and suitable for the quality control of Bisacodyl enteric-coated tablet.