OBJECTIVE： To establish a method for the concentration determination of catecholamines in human urine. METHODS： After extracted by aluminum oxide， using 3， 4-dihydroxybenzylamine as internal standard， HPLC-electrochemistry was performed on the column of Shim-Pack C18 with mixed solution [every 500 ml mixed solution containing ion-pairing reagents （octane sulfonate） 0.5 mmol， methanol 10 ml， 0.06 mmol/L Di-n-butylamine 50 μl， sodium acetate anhydrous 2.0 g， citric acid 5.5 g， EDTA-Na2 60 mg and sodium chloride 584.4 mg， pH＝3.5] at a flow rate of 0.8 ml/min， column temperature was 30 ℃， detection voltage was 750 mV， and injection volume was 40 μl. RESULTS： The linear ranges were 3.125-100 ng/ml for both norepinephrine （r＝0.998 5） and epinephrine （r＝0.999 2）， lower limits of quantitation were 3.125 ng/ml； RSDs of within days and daytime were lower than 10％， method recoveries were 99.2％-101.6％ and 98.9％-100.5％， extraction recoveries were 63.8％-66.8％ and 60.5％-62.6％. CONCLUSIONS： The method shows strong specificity， high sensitivity and low detection cost， and can be used for the concentration determination of catecholamines in human urine.