OBJECTIVE： To establish a method for the simultaneous contents determination of chlorogenic acid， rutin and isoquercitrin in Morus alba， fried M. alba and honeyed M. alba. METHODS： HPLC was performed on the column of Agilent Zorbax SB-C18 with mobile phase of acetonitrile -0.2％ phosphoric acid （gradient elution） at a flow rate of 1.0 ml/min， detection wavelen- gth was 350 nm， the column temperature was 30 ℃，and injection volume was 10 μl. RESULTS： The linear range was 8.20-82.01 μg/ml for chlorogenic acid （r＝0.999 9）， 2.76-27.60 μg/ml for rutin （r＝0.999 9） and 4.74-47.39 μg/ml for isoquercitrin （r＝0.999 9）； RSDs of precision， stability and reproducibility tests were lower than 2％； recoveries were 98.58％-100.91％ （RSD＝1.02％，n＝6）， 99.19％-101.00％ （RSD＝0.82％，n＝6） and 98.41％-101.51％ （RSD＝1.08％，n＝6），respectively. CONCLUSIONS： The method is simple with good stability and reproducibility， and can be used for the simultaneous determination of chlorogenic acid， rutin and isoquercitrin in different processed drugs of M. alba.