OBJECTIVE： To establish the method for the determination of main components content and entrapment efficiency of transferrin modified vincristine-tetrandrine liposomes. METHODS： HPLC method was adopted to determine the content of vincristine. The determination was performed on ELITE C18 column with mobile phase consisted of methanol-15％ triethylamine  （70 ∶ 30） at a flow rate of 1.0 ml/min. The determination wavelength was set at 297 nm and column temperature was 30 ℃. The sample size was 20 μl. Free drug was isolated from liposomes by dextran gel column chromatography， and entrapment efficiency was determined. RESULTS： The linear range of vincristine was 160-1 600 μg/ml （r＝0.999 8， n＝5） with average recovery of 99.20％ （RSD＝0.26％， n＝9）. RSD of precision test was 0.070％ （n＝5）. The average content of vincristine in liposomes was 0.790    mg/ml （RSD＝0.15％， n＝3）， and the average entrapment efficiency was 85.94％ （RSD＝2.08％， n＝3）. CONCLUSIONS： The method is accurate， reliable， simple and rapid， and can be used for the determination of the content and entrapment efficiency of vincristine in transferrin modified vincristine-tetrandrine liposomes.