OBJECTIVE： To establish a method for simultaneous determination of luteolin and apigenin in Mongolian medicine Scabiosa atropurea. METHODS： HPLC was performed on the column of Diamond C18 with mobile phase of acetonitrile-0.4％ phosphoric acid （34 ∶ 66，V/V） at a flow rate of 1.0 ml/min，the detection wavelength was 350 nm， column temperature was 30 ℃， and the injection volume was 10 μl. RESULTS： The linear range was 66-396 ng for luteolin （r＝0.999 8） and 93-558 ng for apigenin（r＝0.999 6）； RSDs of precision， stability and reproducibility tests were lower than 2％； recoveries were 98.15％-101.79％ （RSD＝1.42％，n＝6） and 98.66％-104.05％（RSD＝1.81％，n＝6），respectively. CONCLUSIONS： The method is simple with good precise， stability and reproducibility， and can be used for the simultaneous determination of luteolin and apigenin in Mongolian medicine S. atropurea.