OBJECTIVE: To establish the quality standard for Embelia laeta. METHODS: TLC was adopted for the qualitative identification. HPLC was adopted for the content determination of embelin: the column was Waters C18 with mobile phase consisted of methanol-5% methanoic acid (90 ∶ 10,V/V) at a flow rate of 0.9 ml/min, the detection wavelength was 288 nm, the column temperature was 40 ℃,nd the injection volume was 10 μl. RESULTS: The TLC of E. laeta showed clear spots and good separation. The linear range of embelin was 4.012-40.12 μg/ml (r=0.999 4); RSDs of precision, stability and reproducibility tests were lower than 2%; recovery was 96.54%-99.57%(RSD=1.20%,n=6). CONCLUSIONS: The established standard can be used for the quality control of E. laeta.