OBJECTIVE： To provide reference for the training and evaluation for hospital pharmacy teachers. METHODS： 19 pharmacists-in-charge in the pharmacy department of our hospital received training of teaching ability， and theory test and multi-station practical technique test were conducted after training. The items examined in theory test included training rules， the professional knowledge of pharmacy and teaching method； the content of practical technique test included checking prescriptions/medication orders， drug dispensing， medication management， patient education and ADR report. Each teacher’s test included 3 of 5 items above based on different responsibilities. All scores were analyzed and summarized. RESULTS： The score of practical technique test was higher than the score of theory test； teachers’grasp of training rules was the worst in theory test； in practical technique test， the score of medication management was the highest for teachers worked at the post of dispensing； while the score of patient education was higher than the score of ADR report for teachers who were clinical pharmacists， the differences were statistically significant （P＜0.05）. CONCLUSIONS： In order to improve teaching ability，  teachers should receive intensive training on the training rules and the professional knowledge of pharmacy； the multi-station examination mode will be improved in many modes such as increasing stations， standardized test paper and standardized trainees； meanwhile， the young pharmacists should be encouraged for positive commitment to the teaching work and continuously enriched with experience.