OBJECTIVE： To evaluate the quality of solid lipid nanoparticle of the skin extract of Bufobufo gargarzans. METHODS： The morphology of solid lipid nanoparticle of the skin extract of B. gargarzans was observed by TEM. The particle size was determined by laser scattering particle size analyzer. The contents of cinobufagin and resibufogenin， encapsulation efficiency， drug-loading amount and accumulative release rate of cinobufagin were determined by HPLC. The stability of nanoparticle was investigated within 24 h at 60， 25 and 4 ℃. RESULTS： The solid lipid nanoparticle of the skin extract of B. gargarzans were uniform in particle size and showed round and spheroidicity shape； average particle size was （138.5±4.2） nm， The encapsulation efficiency of cinobufagin and resibufogenin were 90.60％ and 91.51％， and drug-loading amount were 35.82％ and 44.15％. The accumulative release rate of cinobufagin was 50％ at 4 h and reached  88％ at 48 h， which was in line with Weibull equation （r＝0.943 8）. Under 3 kinds of temperature conditions， encapsulation efficiency decreased gradually as the holding time of nanoparticle prolonged； the decrease degree was the smallest at 4 ℃.CONCLUSIONS： The quality evaluation results of solid lipid nanoparticle of the skin extract of B. gargarzans are in line with the standard， and prepared nanoparticles show sustained-release effects and should be kept under low temperature.