OBJECTIVE：To establish the HPLC fingerprint of Lycium ruthenicum and its falsity Nitraria tangutorum. METHODS：HPLC was performed on the column of Diamonsil ODS with mobile phase of acetonitrile-water（gradient elution） at a flow rate of 0.8 ml/min， detection wavelength was 270 nm， column temperature was 30 ℃， and the injection volume was 10 μl. With the references of rutin， TCM chromatographic fingerprint similarity evaluation system was used for the similarity， principal component and cluster analysis of 13 batches of L. ruthenicum and 4 batches of N. tangutorum. RESULTS： There were totally 20 common peaks in 13 batches of L.ruthenicum， similarity of the 12 batches L. ruthenicum of higher than 0.9， which was significantly higher than the similarity of 4 batches of adulterants. 13 batches of L. ruthenicum was in a densely populated area， and the 4 batches of adulterants distributed outside this region. 13 batches of L. ruthenicum clustered together， and 4 batches of adulterants into one group； further cluster analysis was used for the quality evaluation of 13 batches of L. ruthenicum， total clustered into four categories. CONCLUSIONS： The established fingerprint can provide reference for identification and quality evaluation of L. ruthenicum.