OBJECTIVE： To study the tissue distribution regularity of berberine hydrochloride in rats after intragastric administration of Qinglian ningxin capsules. METHODS： 36 rats were given Qinglian ningxin capsules （4 g/kg） intragastrically， and the heart， liver， spleen， lung， kidney tissues were collected 1， 3， 5， 7， 9， 11 h after medication， 6 rats each. Other 6 rats were seleted to collect blank tissue as control. The concentration of berberine hydrochloride in each tissue was determined by HPLC. Zorbax Eclipse XDB- C18 column was used with mobile phase consisted of acetonitrile-0.05 mol/l potassium dihydrogen phosphate （25 ∶ 75， pH＝3.17） at the flow rate of 1.0 ml/min. The detection wavelength was set at 345 nm and column temperature was 25 ℃. RESULTS： The linear range of berberine hydrochloride in each tissue were 0.404 8-404.800 0 μg/ml （r＝0.999 5-0.999 7）； method recovery was 90％-102％ （all RSDs≤6.49％， n＝6） and extraction recovery was 87％-101％ （all RSDs≤5.45％， n＝6）. 1 h after medication， berberine hydrochloride was found in each tissue， and its concentration was the highest in liver. The concentration of berberine hydrochloride in heart reached the maximum 3 h after medication； that in kidney reached the maximum 5 h after medication； that in liver was increasing and reached the maximum 7， 9 and 11 h after medication； that in liver reached 365.263 4 μg/ml 11 h after medication. CONCLUSIONS： Berberine hydrochloride can be delivered in each tissue of rats rapidly after intragastric administration of Qinglian ningxin capsules. The distribation of it is higher and faster in liver and heart.