OBJECTIVE：To establish a method for simultaneous determination of notoginsenoside R1， ginsenoside Rg1 and ginsenoside Rb1 in Shenqi xinshu capsule. METHODS：HPLC was performed on the column of Zorbax SB-C18（150×4.6 mm， 5 μm） with mobile phase of acetonitrile -water at a flow rate of 1.0 ml/min， detection wavelength was 203 nm， column temperature was 30 ℃， and the injection volume was 10 μl. RESULTS：The linear range was 0.199 8-3.996 0 μg for notoginsenoside R1，0.842 8-10.143 0 μg for ginsenoside Rg1 and 0.823 4-9.978 0 μg for ginsenoside Rb1；RSDs of precision， stability and reproducibility tests were lower than 2％； recoveries were 95.17％-100.17％（RSD＝1.81％，n＝9），97.32％-101.18％（RSD＝1.44％，n＝9） and 95.22％-98.89％（RSD＝1.22％，n＝9）. CONCLUSIONS：The method is simple， accurate and reproducible， and can be used for the simultaneous contents determination of notoginsenoside R1， ginsenoside Rg1 and ginsenoside Rb1 in Shenqi xinshu capsule.