OBJECTIVE：To establish a method for content and dissolution determination of berberine hydrochloride in Fengshi qutong capsule. METHODS：HPLC was conducted to determine the content of berberine hydrochloride in the preparation： the column was Waters Xbridge C18 with mobile phase of acetonitrile-0.05 mol/L sodium hydrogen （45 ∶ 55，V/V） at a flow rate of 0.5 ml/min， detection wavelength was 345 nm， column temperature was 30 ℃， and the injection volume was 10 μl. Rotating basket method was used to determine the dissolution of berberine hydrochloride. RESULTS：The linear range of berberine hydrochloride was 1.06-74.2 μg/ml（r＝0.999 9）； RSDs of precision， stability and reproducibility tests were lower than 1％； recovery was 96.8％-100.6％ （RSD＝1.6％，n＝9）. The dissolution of samples in 30 min was higher than 80％. CONCLUSIONS： The method is simple and stable with good reproducibility， and can be used for content and dissolution determination of berberine hydrochloride in Fengshi qutong capsule.