OBJECTIVE： To study the protective effect of Danqing granules on liver function of rats with acute intrahepatic cholestasis and its mechanism. METHODS： 60 rats were randomly divided into blank control group （normal saline）， model group 1 （normal saline）， model group 2 （normal saline）， Yinzhihuang granules group （positive drug， 1.62 g/kg）， Danqing granules high-dose and low-dose groups （6.48， 1.62 g/kg）， with 10 rats in each group. Those group were given α-naphthylisothiocyanate intragastrically to induce acute intrahepatic cholestasis model except normal control group. 48 h after modeling， the rats of model group 1 were sacrificed， and other groups were given relevant medicine intragastrically on the day of modeling， once a day， for consecutive 7 d. The levels of biochemical indexes in serum （ALT， AST， TBIL， DBIL， ALP and TBA）， 3 h bile flow and flow velocity， the concentration of LP-X in serum， mRNA expression of Na+-H+ permutoid were detected in rats. RESULTS： Compared with blank control group， the biochemical indexes and LP-X concentration increased in model group 1 and model group 2， while 3 h bile flow and flow velocity and mRNA expression of Na+-H+ permutoid decreased； and the change of each index in model group 1 were more significant than in model group 2 （P＜0.05）. Compared with model group 2， above indexes of treatment groups were all improved significantly （P＜0.05）. CONCLUSIONS： Danqing granules have positive effect on liver function of acute intrahepatic cholestasis rats， and its mechanism may be associated with up-regulation of mRNA expression of Na+-H+ permutoid.