OBJECTIVE： To optimize ultrasonic extraction and purification technology of solanesol from tobacco leaf. METHODS： Using extraction rate and transport rate of solanesol as indexes， single factor test was used to investigate liquid-solid ratio， ultrasonic extraction temperature and time， ultrasonic power and extraction times， and the amount of soap alkali lye （volume ratio of soap alkali lye to extraction liquid）， acidizing fluids （volume ratio of acidizing fluids to soap alkali lye extract）， extraction times of purification technology. Optimized technology was validated， and the purity of solanesol was calculated； the amount of extracted solanesol was compared between this method and traditional extraction method （spending 30 h）， solvent continuous cyclic extraction （spending 5-6 h）. RESULTS： Optimized extraction technology was as follows as volume ratio of soap alkali lye to extraction liquid 1 ∶ 14， ultrasonic extraction temperature 70 ℃， ultrasonic extraction time 60 min， ultrasonic power 120 W， extracting for 3 times； optimized purification technology was as follows as volume ratio of soap alkali lye to extraction liquid 2 ∶ 35， volume ratio of acidizing fluids to soap alkali lye extract 2 ∶ 14， extracting for 4 times. In validation test， extraction rate， transport rate and purity were 92.45％ （RSD＝0.46％， n＝3）， 79.88％ （RSD＝0.30％， n＝3） and 55.86％ （RSD＝0.40％， n＝3）. The amount of solanesol extracted with 3 methods were 52.22， 45.22 and 26.10 mg/g. CONCLUSIONS： The optimized technology is simple and stable， costs less time and saves source with high extraction amount and purity， which is suitable for production， extraction and purification of solanesol from tobacco leaf.