OBJECTIVE： To establish the quality standard for Gentiana scabra dispensing granule. METHODS： TLC was adopted to identify G. scabra in the preparation； HPLC was adopted to determine the content of gentiopicroside in the preparation： the column was Dionex C18 with mobile phase of methanol - water （25 ∶ 75，V/V） at a flow rate of 1.0 ml/min， the detection wavelength was 270 nm， column temperature was 30 ℃， the injection volume was 10 μl. RESULTS： TLC showed clear spots and good separation. The linear range of gentiopicroside injection volume was 0.302 6-3.026 μg （r＝0.999 9）； RSDs of precision， stability and reproducibility tests were lower than 2％； recovery was 98.27％-99.56％ （RSD＝0.68％，n＝6）. CONCLUSIONS： The established standard can be used for the quality control of Gentiana scabra dispensing granule.