OBJECTIVE： To investigate toxic reaction of Compound zedoary turmeric oil cream in experimental rats with long-term consecutive transdermal administration， and to provide reference for safe use of it in the clinic. METHODS： 60 SD rats were randomly divided into blank control （cream matrix） group， Compound zedoary turmeric oil cream intact skin and damaged skin low-dose and high-dose （5％， 10％） groups， with 12 rats in each group， half male and half female. All of them were given relevant medicine twice a day. 92 d consecutive medication later， general situation of rats were observed， and body weight， blood routine （WBC， RBC， HGB， LYMPH， etc.） and blood biochemical indicators （AST， ALT， PA， etc.） were all detected； systematical observation of organs， organ coefficient calculation and histopathology examination were carried out. RESULTS： There was no statistical significance in those indicators between Compound zedoary turmeric oil cream groups and blank control group （P＞0.05）， except hemoglobin decreased in intact skin low-dose group， while hemoglobin decreased， LYMPH and PA increased in damaged skin high-dose group （P＜0.05）. Pathology results showed that Compound zedoary turmeric oil cream had no significant toxicity for the main organs. CONCLUSIONS： Compound zedoary turmeric oil cream has no long-term toxicity to experimental rats.