OBJECTIVE： To study antibacterial effect of Hushi enema granules in vitro. METHODS： Minimal inhibitory concentration （MIC） of Hushi enema granules [initial concentration of 1 200 mg （crude drug）/ml] to Staphylococcus aureus， Escherichia coli， Salmonella， Shigella dysenteriae and Shigella flexneri were detected by tube double dilution method. The minimal bactericidal concentration （MBC） were determined by agar medium plate method； 4 MIC and 2 MIC Hushi enema granules solution were selected to determine sterilizing speed （bacterium concentration of 1.5×105 CFU/ml） and draw time-kill curves （KCS）.  RESULTS： MIC of Hushi enema granules to S. aureus， E. coli， Salmonella， S. dysenteriae and S. flexneri were 18.75， 150， 150， 75 and 150 mg（crude drug）/ml， respectively； MBC of Hushi enema granules were 37.50， 150， 150， 75 and 150 mg（crude drug）/ml， respectively. Both 4 MIC and 2 MIC solution had good sterilizing effects on 5 bacterial strains. KCs of 4 MIC and 2 MIC solution sterilizing 5 bacterial strains were 24， 1， 1， 7， 1 h and 24， 7， 3， 7， 7 h， respectively.  CONCLUSIONS： Hushi enema granules show a strong antibacterial effect in vitro.