OBJECTIVE：To compare the different of contents of the active ingredient baicalin in Scutellaria baicalensis granule preparation and decoction piece. METHODS： HPLC was performed on the column of Waters C18 with mobile phase of methanol -0.3％ acetic acid （47 ∶ 53，V/V） at a flow rate of 0.8 ml/min， the detection wavelength was 280 nm， the column temperature was 25 ℃， and the injection volume was 20 μl. RESULTS： The linear range of baicalin was 10-100 μg/ml （r＝0.999 9）； RSDs of precision， stability and reproducibility tests were lower than 2％； recoveries were 95.48％-98.98％（RSD＝1.26％，n＝6， decoction piece） and 95.76％-100.50％ （RSD＝1.75％，n＝6， granule）. CONCLUSIONS：The method is simple， stable and reproducible， and can be used for the content determination of baicalin in S. baicalensis granule preparation and decoction piece. There is no discernible differences between each kind of S. baicalensis granule preparation and decoction piece.