OBJECTIVE： To optimize the extraction technology of nucleoside from Codonopsis pilosula. METHODS： The ultrasonic extraction was used to extract nucleoside from C. pilosula. The extraction technology was screened and optimized by single factor and orthogonal test with ethanol concentration （including water）， extraction time， solid-liquid ratio and extraction times as factors using the total contents of cytidine， uridine， guanosine and adenosine as index. Validation test was conducted. RESULTS： The optimal extraction technology was that pure water was extraction solvent， extracting for 45 min， with material-liquid ratio of 1 ∶ 20 （g/ml）， extracting for 2 times. Total amount of 4 kinds of nucleosides from in 100 g C. pilosula was （17.01±0.005） mg （RSD＝0.68％， n＝3）. CONCLUSIONS： Optimized extraction technology of nucleoside from C. pilosula is stable， feasible， rapid in operation and cost-saving.