OBJECTIVE： To study the protective effects of endotoxin adsorbent SPV on intestinal mucosa injury in rats with traumatic and hemorrhagic shock. METHODS： 0.5 mg polymyxin B， 5 mg vitamine B6 and 0.3 g Montmorillonite powder were dissolved in normal saline to prepare 5 ml solution， i.e. low-dose of SPV. Medium-dose and high-dose drug were 2 and 3 folds of low- dose. Rats were randomly divided into normal control group， model group and SPV low-dose， medium-dose and high-dose groups with 10 rats in each group. Except for normal control group， other groups were given by femoral artery bloodletting to induce traumatic and hemorrhagic shock model. 30 min before modeling， rats of SPV groups were given relevant dose of SPV intragastrically. 1， 4， 8 and 16 h after resuscitation， the serum levels of NO， DAO， endotoxin and TNF-α were detected in all rats. The intestinal mucosa cells characteristics and intestinal mucosa lesion index were observed in normal control group， model group and SPV high-dose group. RESULTS： Compared with normal control group， intestinal mucosa cells necrosis of model group was found， and the serum levels of NO， DAO， endotoxin and TNF-α increased （P＜0.05）. Compared with model group， intestinal mucosa cells injury relieved significantly in SPV high-dose and medium-dose groups 1， 4， 8 and 16 h after resuscitation， and the serum levels of NO， DAO， endotoxin and TNF-α decreased （P＜0.05）； there was no significant difference of the other groups（P＞0.05）. CONCLUSIONS： The endotoxin adsorbent SPV can significantly reduce the serum levels of NO， DAO， endotoxin and TNF-α in rats with traumatic and hemorrhagic shock， it has protective effect on intestinal mucosal injury induced by traumatic and hemorrhagic shock.