OBJECTIVE： To study the induction effect of ent-kaurane diterpenoid Leukamenin E on the differentiation of human promyelocytic leukemia HL-60 cells. METHODS： Trypan blue staining， Giemsa’s staining， NBT reduction test， fluorescent particles phagocytosis test and flow cytometry were used to detect the number of HL-60 cells after treated with 0， 0.3， 0.6， 0.9 and 1.2 μmol/L Leukamenin E and 1.2 μmol/L all-trans retinoic acid （ATRA） for 24，48，72 and 96 h， and the cellular nucleus morphology， NBT-reducing ability （by NBT positive cell rate）， phagocytic ability （by fluorescence intensity of fluorescence probe P） and the expression of antigen CD11b after treated for 72 h were observed. RESULTS： Compared with blank control， the number of HL-60 cells decreased after treated with 0.3-1.2 μmol/L Leukamenin E and 1.2 μmol/L ATRA for 48， 72 and 96 h； the number of band nucleus and segmented nuclei increased after treated for 72 h （P＜0.01）； the rate of positive NBT cells and the expression of CD11b increased （P＜0.01）；  the fluorescence intensity of P strengthened. CONCLUSIONS： Leukamenin E could induce the differentiation of HL-60 cells to mature granulocyte， which is similar to the effects on HL-60 cells induced by leukemia differentiation therapeutic agent ATRA.