OBJECTIVE： To investigate the sensitivity and drug resistance of Helicobacter pylori （Hp） clinical isolated to commonly used antibiotics in Chongqing area， and to analyze the reasons for drug resistance. METHODS： Hp strains were collected from gastroscope room of 4 hospitals from Chongqing area during Apr. to Dec. 2014， and gastric biopsy samples were randomly collected from 189 patients. Isolated Hp strains were cultured and used for antimicrobial susceptibility test in vitro. The sensitivity of Hp to metronidazole， clarithromycin， amoxicillin， levofloxacin， tetracycline and furazolidone were determined and compared with results in year of 2005. At the same time， questionnaire survey was conducted among physicians about their antibacterial choice in Hp treatment. RESULTS： A total of 63 Hp strains were cultured. Of these strains， 100％ were resistant to metronidazole， 28.6％ were resistant to clarithromycin， and 34.9％ were resistant to tetracycline. Resistance to amoxicillin， furazolidone or levofloxacin were not found in the study. Notably， 28.6％ of the isolates were found to be resistant to both metronidazole and clarithromycin； resistance to metronidazole had increased in 2005， while its resistance to furazolidone decreased， with statistical significance （P＜0.05）. In questionnaire survey， physicans favored to use amoxicillin+clarithromycin in Hp treatment. CONCLUSIONS： In general， resistance to metronidazole， levofloxacin and clarithromycin are higher， which could be due to physicians’ choice and to the frequent use of this antibiotics. These results suggest that amoxicillin， furazolidone or tetracycline is better choice in the treatment of Hp infections in Chongqing area.