OBJECTIVE：To establish a method for the simultaneous determination of gallic acid， rhein and emodin in Compound xiaozhi suppository. METHODS：HPLC was performed on the column of Kromasil C18 with mobile phase of 0.5％ phosphoric acid-Acetonitrile solution（gradient elution） at a flow rate of 1.0 ml/min， the detection wavelength was 273 nm for gallic acid and 254 nm for rhein and emodin， the column temperature was 28 ℃， and the injection volume was 20 μl. RESULTS：The linear range was 0.296 4-14.82 μg/ml for gallic acid（r＝0.999 6），0.215 0-10.75 μg/ml for rhein（r＝0.999 9） and 0.307 2-15.36 μg/ml for emodin（r＝0.999 9）；RSDs of precision， stability and reroducibility tests were lower than 3.0％； recoveries were 95.1％-97.2％ （RSD＝0.64％，n＝6），95.4％-97.2％（RSD＝0.42％，n＝6） and 96.5％-99.4％（RSD＝1.10％，n＝6）， respectively. CONCLUSIONS：The method is simple and accurate， and can be used for the contents determination of gallic acid， rhein and emodin in Compound xiaozhi suppository.