OBJECTIVE：To understand the main diseases research area and tendency of the literatures published on top-five global biomedicine journals， and provide reference for topics and designs of domestic medical research. METHODS： Studies published on NEJM， JAMA， BMJ， Lancet and PLoS Medicine from 2004 to 2013 were searched via Web of Science； types of disease were classified by using International Classification of Diseases （version 10） （ICD-10）； research types were analyzed according to the rules in clinical epidemiology. RESULTS： Most of studies （25.8％） were published on BMJ； USA （39.1％）， UK （25.7％） and Canada （5.1％） were top-three countries for the first authors； circulation system disease， infectious and parasitic diseases， cancer， endocrine， nutritional and metabolic disease and respiratory system disease were top-five in disease categories list， accounting for 61.7％； ischemic heart disease， HIV-related disease， diabetes， other heart disease and influenza and pneumonia were the main top-five specific diseases； and randomized controlled trials were main study type. CONCLUSIONS： The proportion of the first author for the Chinese literature in the host country is very low. Top-five specific diseases are placed on circulation system disease， infectious and parasitic diseases， endocrine， nutritional and metabolic disease and respiratory system disease， with the main study types of randomized controlled trial， cohort study and systematic review.