OBJECTIVE： To analyze blood concentration monitoring data of digoxin in Beijing Hospital （called “our hospital” for short） and its influential factors， and to provide reference for rational use of drugs in the clinic. METHODS： 3 385 patients underwent blood concentration monitoring of digoxin were selected from our hospital during 1996-2015. Blood concentration of digoxin was detected by fluorescence polarization immunoassay. The effects of patients’ gender and age， drug dose and other factors on blood concentration were analyzed. RESULTS： The rate of digoxin medication monitoring in our hospital decreased from 78.9％ in 2002 to 37.2％ in 2015， showing a descending tendency. Results of 3 385 times monitoring showed that average blood concentration of digoxin was （1.05±0.04）ng/ml； blood concentration of 2 391 cases were in normal range （0.5-1.75 ng/ml）， accounting for 70.64％. Average blood concentration of digoxin in female patients [（1.06±0.04） ng/ml] was slightly higher than in male patients [（1.05±0.04） ng/ml]， without statistical significance （P＞0.05）. Most of patients were aged ≥70 years， and patients’ age was positively correlated to blood concentration [relative coefficient （r） were 0.923 and 0.958， both P＝0.000 2]； drug dose was not correlated with blood concentration （r＝0.773， P＝0.125）. CONCLUSIONS： The application of digoxin become more and more reasonable in our hospital， and its blood concentration is influenced by patients’ age. Individual therapy regimens should be formulated according to blood concentration monitoring and clinical symptom of patients.