OBJECTIVE：To establish a method for the simultaneous determination of salvianolic acid B， puerarin and hydroxysafflor yellow A in Xinshule tablet. METHODS： HPLC was performed on a Welchrom C18 with mobile phase of acetonitrile - 0.1％ phosphoric acid （gradient elution） at a flow rate of 1.0ml/min， detection wavelength was 280 nm， column temperature was 35 ℃， and injection volume was 10 μl. RESULTS： The linear range was 31.54-189.22 μg/ml for salvianolic acid B （r＝0.999 1）， 17.13-102.76 μg/ml for puerarin（r＝0.999 7） and  4.27-25.60 μg/ml for hydroxysafflor yellow A（r＝0.999 5）； RSDs of precision， stability and reproducibility tests were lower than 2％； recoveries were 96.29％-100.88％ （RSD＝1.73％，n＝6）， 97.12％-100.57％ （RSD＝1.15％，n＝6） and 97.09％-99.95％（RSD＝1.01％，n＝6）， respectively. CONCLUSIONS： The method is simple， practical and reproducible， and can effectively control the quality of preparation.