OBJECTIVE：To establish a method for the simultaneous determination of curdione， germacrone and furanodiene of zedoray turmeric oil in Ruxiankang injection. METHODS：HPLC was performed on the column of Zorbax SB-C18 with mobile phase of acetonitrile-water （gradient elution） at a flow rate of 1.0 ml/min， detection wavelength was 216 nm， column temperature was 30 ℃， and the injection volume was 10 μl. RESULTS： The linear range was 60-480 μg/ml for curdione（r＝0.999 0），40-320 μg/ml for germacrone （r＝0.999 0） and 40-320 μg/ml（r＝0.999 0）； RSDs of precision， stability and reproducibility tests were lower than 2.0％； recoveries were 95.21％-99.89％（RSD＝1.6％，n＝6），102.33％-104.89％（RSD＝1.0％，n＝6） and 97.38％-99.06％ （RSD＝0.7％，n＝6）， respectively. CONCLUSIONS： The method is simple and accurate， and can be used for simultaneous contents determination of curdione， germacrone and furanodiene of zedoray turmeric oil in Ruxiankang injection.