OBJECTIVE： To provide reference for rational use of antibiotics in the clinic. METHODS： The application of antibiotics in our hospital during 2010-2014 was analyzed by DDD analysis， and bacterial resistance monitoring data announced by bacterial office of laboratory medicine department were summarized and correlation was analyzed by statistical analysis software. RESULTS： From 2010 to 2014， the total DDDs of antibiotics down to the peak valley in 2012， then stepped up year by year till 2014 to reach the highest. DDDs of Ⅱ generation cephalosporin were at the top during 5 years， and its constituent ration ranged 24.93％-31.42％. The major isolated bacterial was gram-negative bacterial， among which constituent ratio of Escherichia coli， Pseudomonas aeruginosa and Klebsiella pneumoniae ranked the top 3. DDDs of cefuroxime took up the first place during 2011-2014. K. pneumoniae kept a low resistance to most antibiotics， while Acinetobacter baumannii was getting worse. DDDs of Ⅱ generation cephalosporin had the significant positive correlation with the resistance rate of E. coli to amoxicillin and clavulanate potassium （P＜0.05）. DDDs of macrolide had the significant negative correlation with the resistance rate of E. coli to piperacillin-tazobactam and amikacin（P＜0.05）. The resistance rate of K. pneumoniae to piperacillin had the significant negative correlation with DDDs of Ⅱ generation cephalosporin （P＜0.05）， while to piperacillin-tazobactam had the significant negative correlation with DDDs of Ⅲ generation cephalosporin （P＜0.05）. DDDs of Ⅲ generation cephalosporin had the significant positive correlation with the resistance rate of A. baumannii to ceftazidime， cefepime， piperacillin sodium and tazobactam sodium， imipenem， ciprofloxacin（P＜0.05）. CONCLUSIONS： Resistance rate of bacterial is correlated to DDDs of antibiotics. drug resistance of A. baumannii ia severe， and unreasonable use of antibiotics still exist in our hospital， and it is necessary to control the use of Ⅱ and Ⅲ generation cephalosporin.