OBJECTIVE：To investigate penetrative effects of a penetration enhancer and multiple penetration enhancers combination with different proportions on Euodia rutaecarpa superfines cataplasm，so as to optimize enhancer and their concentrations. METHODS：Modified Franz diffusion cell was employed with isolated mice abdominal skin as barrier. HPLC method was adopted to detect the effects of a penetration enhancer （propanediol，azone，oleic acid），multiple penetration enhancers （propanediol- azone，propanediol-oleic acid，propanediol-azone-oleic acid），their proportion and amount on accumulative permeation quantity （Qn）of evodiamine and rutaecarpin in Euodia rutaecarpa superfines cataplasm. RESULTS：The penetrative effect of a penetration enhancer propanediol was significantly better than that of other one penetration enhancers and multiple penetration enhancers；the higher proportion of propanediol in multiple penetration enhancer system was，the better penetrative effects of evodiamine and rutaecarpin had. Using 3％，5 ％ and 7 ％ propanediol as enhancer，Q36 h of evodiamine were 11.290，14.332 and 13.537 μg/cm2，and those of rutaecarpin were 11.965，14.856 and 13.901 μg/cm2. CONCLUSIONS：The penetrative effect of 5％ propanediol is the best，and can be used as enhancer for E. rutaecarpa superfines cataplasm.