OBJECTIVE：To establish a method for the limit detection of aconitine in Wuqi shujin tongluo tablet. METHODS： TLC was conducted to identify the aconitine；TLC plate was silica gel G plate，developing solvent was toluene-ethyl acetate-chloroform- acetone-ammonia（20 ∶18 ∶3 ∶6 ∶1，V/V/V/V/V），chromogenic agent was bismuth potassium iodide test solution and sodium nitrite ethanol test solution；and durability investigation and detection limit detection were used to optimize the TLC plate，temperature and humidity. RESULTS：TLC showed the aconitine had clear spots and negative control without interference. The durability was good；detection limit was 0.9 μ g；available TLC plate was Merck HPTLC prefabricated plate，silica gel G TLC plate setf-made silica gel G TLC plate with adhesive of sodium carboxymethycellulose；temperature was 5-16 ℃ and humidity was 32％-72％. CONCLUSIONS：The method is simple and reproducibility，and can be used for the limit detection of aconitine in Wuqi shujin tongluo tablet.