OBJECTIVE： To provide reference for clinical infection control and experiential therapy. METHODS： From 2006 to 2011， Mohnarin cerebrospinal fluid （CSF） isolates reports and literatures were collected and analyzed statistically by using SPSS.19.0 software. RESULTS： During 2006-2011， Gram-positive bacteria in CSF isolates were on the rise， and Gram-negative bacteria were decreased， with statistical significance （χ2＝12.952，P＝0.01）. Resistance rate of Staphylococcus showed a significant decrease trend， with statistical significance （P＜0.001）， especially to Erythromycin. Resistance rate of Staphylococcus to levofloxacin and ciprofloxacin had declined slightly， but there was no statistical significance （P＞0.05）. That Staphylococcus aureus resistant to vancomycin and linezolid had not been found. Resistance rate of Enterococcus to vancomycin increased significantly， with statistical significance （P＜0.001）； to levofloxacin， there was no statistical significance （P＞0.05）； linezolid-resistant strains was not found. Resistance rate of Gram-negative bacterial to antibiotics increased gradually year by year， especially multiple drug resistance of Acinetobacter； that of Acinetobacter to commonly used antibiotics increased significantly， with statistical significance （P＜0.001）. CONCLUSIONS： Among CSF clinical isolates， Staphylococcus epidermis and Acinetobacter are increasing year by year； drug resistance of Gram-positive bacteria show a downward trend， while Gram-negative bacteria tend to increase significantly.