OBJECTIVE： To study the effect of Typhae pollen decoction on isolated uterine smooth muscle （USM） contraction of rats at postconceptual stage. METHODS： 16 Wistar rats at postconceptual stage were selected and USM strips were isolated. T. pollen decoction 0 （blank control）， 2， 4， 8 and 12 mg （medicinal material）/ml were added respectively； the effects of T. pollen decoction on USM strips contraction were observed， and mean tension， duration of contraction and frequency were measured and recorded. The effects of T. pollen decoction on USM strips contraction were investigated after pretreatment of prostaglandin synthase inhibitor indometacin （1×10－7 mol/ml） and calcium channel blocker verapamil （1×10－7 mol/ml）； USM tension， duration of contraction and frequency were measured and recorded. RESULTS： Compared with blank control group， T. pollen decoction 4， 8 and 12 mg （medicinal material）/ml could increase USM tension， duration of contraction and frequency （P＜0.05）； after added verapamil and indometacin， T. pollen decoction decreased USM tension， shortened duration of contraction and decreased frequency （P＜0.05）. CONCLUSIONS： T. pollen decoction can strengthen USM strips contraction， and the effect is inhibited by prostaglandin synthase inhibitor and calcium channel blocker.