OBJECTIVE：To establish a method for simultaneous determination of geniposide，berberine hydrochloride and baicalin in Annao pill. METHODS：HPLC was performed on the column of Inertsil ODS-3 C18 with mobile phase of acetonitrile - 0.2％ phosphoric acid solution（gradient elution）at a flow rate of 1.0 ml/min，the column temperature was 30 ℃，the detection wavelength was 230 nm，and the injection volume was 10 μl. RESULTS：The linear range was 0. 041 0-0.820 0 μg for geniposide （r＝0.999 9），0.037 6-0.752 0 μg for berberine hydrochloride（r＝0.999 9）and 0.095 2-1.904 0 μg for baicalin（r＝0.999 9），respectively； recoveries were 97.31％ -100.41％（RSD＝1.10％ ，n＝6），98.32％ -101.76％（RSD＝1.20％ ，n＝6） and 99.16％ -101.30％ （RSD＝0.76％，n＝6），respectively. CONCLUSIONS：The method is simple and accurate，and can be used for the quality control of Annao pill.