OBJECTIVE： To explore the intervention effects by clinical pharmacist on the use of antibacterials during perioperative period of hysteroscopic surgery. METHODS： 250 medical records of hysteroscopic surgery were collected from July to September in 2014 （before intervention） and from October to December in 2014 （after intervention）. The rationality of antibacterials during perioperative period was retrospectively analyzed before and after intervention. RESULTS： After intervention by clinical pharmacists， the antibacterial use density decreased from 47.02 DDDs/（100 persons·day） to 23.30 DDDs/（100 persons·day）， and the rate of perioperative use of antibacterial decreased from 82.40％ to 57.20％， with significant difference（P＜0.01）. The rationality of medication indicators for perioperative application of antibacterials for hysteroscope increased from 56.80％ to 82.52％； the rationality of medication duration increased from 49.51％ to 79.02％， with significant difference（P＜0.05）. CONCLUSIONS： Clinical pharmacist’s intervention is effective and feasible to perioperative application of antibacterials for hysteroscopic surgery. It could effectively promote the rational use of antibacterials.