OBJECTIVE： To observe the effect of room temperature and low temperature storage on the chronergy of domestic cisatracurium. METHODS：90 patients undergoing elective surgery under general anesthesia were randomly divided into low temperature group，room temperature A group and room temperature B group，with 30 cases in each groups. Through single supramaximal electrical stimulation of the adductor pollicis muscle，TOF-Watch SX muscle relaxation monitor was used to observe the degree of muscle relaxation. After induction of anesthesia and lost consciousness，the patients was given intravenous injection of cisatracurium 0.2 mg/kg；when muscle twitch decreased to the maximum inhibition degree，the patient received endotracheal intubation. The cisatracurium of low temperature group was stored in refrigerator at 2 to 5 ℃；that of room temperature A group and room temperature B group was stored in incubator for 15 days or 30 days. The onset time，maximum inhibition degree of muscle twitch，clinical duration，recovery index and total chronergy were recorded in 3 groups. RESULTS：The maximal inhibition degree of muscle twitch reached 0 under the action of cisatracurium. There was no statistical significance in onset time and clinical duration between room temperature A group，room temperature B group and low temperature group（P＞0.05）；the recovery index and total chronergy of room temperature A group and room temperature B group were or longer than low temperature group，with statistical significance（ P＜0.05）. CONCLUSIONS：Domestic cisatracurium stored at low temperature shows weaker effect on muscle relaxation.