OBJECTIVE： To provide reference for the application of micro-lecture in pharmacology teaching. METHODS： Based on a correct view of micro-lecture and the sample of positive inotropic effect and mechanism of cardiac glycosides， micro-lecture design of pharmacology was explored from aspects of design objects，contents，technical support and application mode. RESULTS： As the main carrier of micro-lecture， video took the advantages of topic prominence， resource diversity， short and pithy and easy to use； the objects were five-year undergraduate medical students of the Jiangxi Univesity of Traditional Chinese Medicane； and the key and difficult points in pharmacology courses were selected for the complete teaching of these points in a short time as much as possible； record screen-based software model was conduced for video making and the course was uploaded to Kingsoft disk sharing. CONCLUSIONS： As a new teaching mode， course design is the basis and it is essential for the smooth development. A good micro-lecture design has positive improvement effect on improving the teaching efficacy of Pharmacolgy traditional class.